FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_70_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_70_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19572599
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG87500.017.1555881
CCTAGAC70950.017.004793
TATACTG116900.016.5374575
TATACCG61900.016.4214675
GTATTGG159000.015.9540411
TAGACTG64150.015.4752765
TACACTG94150.015.437965
CCTATAC90950.015.0411033
GTATAAG84200.014.7249881
TAGACAG75000.014.69317155
CTATACT123000.014.5202024
TATACAG108950.014.3435775
TACACCG75250.014.2656225
CCTACAC87700.014.2444623
GTCTAGG79350.013.8888911
GTGTAGG93000.013.8424471
GTATAGA101450.013.6727961
TACACAG89000.013.60945
ACTGTGC96000.013.556838
TATTGGG177400.013.3879172
Produced by FastQC (version 0.11.5)