FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_120_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_120_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17149138
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG81350.016.9340381
TATACTG99550.015.3161545
TAGACTG55000.015.1997315
CCTATAC80600.014.6741013
GTATATA204900.014.1419091
GTATTGG123350.013.7483061
TACACTG80000.013.6560095
GTCTAGG56800.013.6319941
TATACAG98800.013.5093775
ACTGTGC82600.013.4559838
CTATACT103700.013.3749234
ATTATAC102000.013.2252963
TACACAG77350.013.0799245
GACTGTG54000.012.8423297
GTACCGT51250.012.5119746
ACAGTGC89050.012.481358
TCTATAC75050.012.4048813
GTGTAAG50000.012.3507551
TAATACT115050.012.3444514
TTATACT116300.012.3342994
Produced by FastQC (version 0.11.5)