FastQCFastQC Report
Fri 22 Jul 2022
HT5W2AFX3_n02_12016-e-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5W2AFX3_n02_12016-e-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3197597
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80849925.284580889962054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG40270.1259383218085331No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG39450.1233738960850914No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG36600.11446095302190988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG33640.10520400162997402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG33320.1042032501281431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)