FastQCFastQC Report
Fri 22 Jul 2022
HT5W2AFX3_n01_12016e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5W2AFX3_n01_12016e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666677
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC58648421.99306477687399TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTGTGC165300.6198725979936828TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC106110.39791095809503735TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGATGC55250.20718669715154855TruSeq Adapter, Index 11 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC41120.15419940247731542TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTGTGC35760.1340994803645136TruSeq Adapter, Index 11 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACATCTCGTATGC32730.12273702439403048TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAGATCTCGTATGC30990.11621204967830749TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTATATCTCGTATGC29500.11062457132978609TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATGC28620.10732458411723655TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGGCTACATCTCGTATGC27870.1045120950156318TruSeq Adapter, Index 11 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)