FastQCFastQC Report
Fri 22 Jul 2022
HT5W2AFX3_n01_12016-in-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5W2AFX3_n01_12016-in-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9673698
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1881161.9446131148605217TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1288431.3318898315825034TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC736590.7614358025235024TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATGC650840.6727933826340248TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC505790.522850723683952TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGGATGC323920.3348460950507241TruSeq Adapter, Index 5 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGATGC283040.2925871781401487TruSeq Adapter, Index 5 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC257840.2665371608665063TruSeq Adapter, Index 5 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)