FastQCFastQC Report
Fri 22 Jul 2022
HT5W2AFX3_n01_12012in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5W2AFX3_n01_12012in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101072
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC119513811.831793694768239TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCGATCTCGTATGC110490.10938442969221485TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC109150.10805783782156983TruSeq Adapter, Index 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)