FastQCFastQC Report
Fri 22 Jul 2022
HT5W2AFX3_n01_12012in-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5W2AFX3_n01_12012in-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9114229
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT5997426.580282325581242TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTGT479850.5264844673093029TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT374100.41045709955279813TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTGT225000.2468667399074568TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGGAT191970.21062670248904214TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGGGTGT96590.10597714847849446TruSeq Adapter, Index 20 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)