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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-09-19, 18:09 based on data in: /beegfs/mk5636/logs/html/HT5VLAFXY/merged

        General Statistics

        Showing 118/118 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HT5VLAFXY_n01_13YCol_14YC24_15YCol_16YC24_lib_k
        32.7%
        42%
        1.2
        HT5VLAFXY_n01_13YCol_14YC24_15YCol_16YC24_lib_k-b
        32.7%
        42%
        1.2
        HT5VLAFXY_n01_13YCol_14YC24_lib_k
        53.1%
        40%
        0.0
        HT5VLAFXY_n01_13YCol_14YC24_lib_k-b
        53.1%
        40%
        0.0
        HT5VLAFXY_n01_13YCol_14YCol_15YCol_16YCol_lib_k
        21.0%
        41%
        0.5
        HT5VLAFXY_n01_13YCol_14YCol_15YCol_16YCol_lib_k-b
        21.0%
        41%
        0.5
        HT5VLAFXY_n01_13YCol_14YCol_lib_k
        20.6%
        40%
        2.4
        HT5VLAFXY_n01_13YCol_14YCol_lib_k-b
        20.6%
        40%
        2.4
        HT5VLAFXY_n01_13YCol_Col_lib_k
        31.6%
        42%
        1.8
        HT5VLAFXY_n01_13YCol_Col_lib_k-b
        31.6%
        42%
        1.8
        HT5VLAFXY_n01_14YCol_Col_lib_k
        24.5%
        40%
        3.3
        HT5VLAFXY_n01_14YCol_Col_lib_k-b
        24.5%
        40%
        3.3
        HT5VLAFXY_n01_15YCol_Col_lib_k
        61.8%
        42%
        3.2
        HT5VLAFXY_n01_15YCol_Col_lib_k-b
        61.8%
        42%
        3.2
        HT5VLAFXY_n01_16YCol_Col_lib_k
        24.2%
        39%
        6.0
        HT5VLAFXY_n01_16YCol_Col_lib_k-b
        24.2%
        39%
        6.0
        HT5VLAFXY_n01_HALObZIP53_pGSTbZIP63_Col_B_k
        31.2%
        40%
        11.2
        HT5VLAFXY_n01_HALObZIP53_pGSTbZIP63_Col_B_k-b
        31.2%
        40%
        11.2
        HT5VLAFXY_n01_HALObZIP63_pGSTbZIP53_Col_B_k
        55.3%
        36%
        0.6
        HT5VLAFXY_n01_HALObZIP63_pGSTbZIP53_Col_B_k-b
        55.3%
        36%
        0.6
        HT5VLAFXY_n01_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k
        65.1%
        40%
        1.6
        HT5VLAFXY_n01_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b
        65.1%
        40%
        1.6
        HT5VLAFXY_n01_SPL9_13YCol_14YC24_Col_B_k
        60.9%
        40%
        1.3
        HT5VLAFXY_n01_SPL9_13YCol_14YC24_Col_B_k-b
        60.9%
        40%
        1.3
        HT5VLAFXY_n01_SPL9_13YCol_14YCol_15YCol_16YCol_B_k
        52.5%
        38%
        2.7
        HT5VLAFXY_n01_SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b
        52.5%
        38%
        2.7
        HT5VLAFXY_n01_SPL9_13YCol_14YCol_Col_B_k
        43.4%
        39%
        0.7
        HT5VLAFXY_n01_SPL9_13YCol_14YCol_Col_B_k-b
        43.4%
        39%
        0.7
        HT5VLAFXY_n01_SPL9_13YCol_Col_B_k
        70.8%
        40%
        0.6
        HT5VLAFXY_n01_SPL9_13YCol_Col_B_k-b
        70.8%
        40%
        0.6
        HT5VLAFXY_n01_SPL9_14YCol_Col_B_k
        62.8%
        38%
        1.8
        HT5VLAFXY_n01_SPL9_14YCol_Col_B_k-b
        62.8%
        38%
        1.8
        HT5VLAFXY_n01_SPL9_15YCol_Col_B_k
        75.5%
        38%
        1.7
        HT5VLAFXY_n01_SPL9_15YCol_Col_B_k-b
        75.5%
        38%
        1.7
        HT5VLAFXY_n01_SPL9_16YCol_Col_B_k
        65.4%
        38%
        2.6
        HT5VLAFXY_n01_SPL9_16YCol_Col_B_k-b
        65.4%
        38%
        2.6
        HT5VLAFXY_n01_bZIP53_Col_B_k
        32.1%
        40%
        11.2
        HT5VLAFXY_n01_bZIP53_Col_B_k-b
        32.1%
        40%
        11.2
        HT5VLAFXY_n01_bZIP63_Col_B_k
        50.2%
        37%
        2.5
        HT5VLAFXY_n01_bZIP63_Col_B_k-b
        50.2%
        37%
        2.5
        HT5VLAFXY_n01_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k
        30.5%
        39%
        17.0
        HT5VLAFXY_n01_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b
        30.5%
        39%
        17.0
        HT5VLAFXY_n01_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k
        42.9%
        36%
        0.9
        HT5VLAFXY_n01_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k-b
        42.9%
        36%
        0.9
        HT5VLAFXY_n01_dDAP_HALO_GST_Col_B_k
        2.3%
        42%
        0.0
        HT5VLAFXY_n01_dDAP_HALO_GST_Col_B_k-b
        28.6%
        39%
        0.9
        HT5VLAFXY_n01_dDAP_HALObZIP53_GSTbZIP63_Col_B_k
        35.8%
        40%
        10.7
        HT5VLAFXY_n01_dDAP_HALObZIP53_GSTbZIP63_Col_B_k-b
        35.8%
        40%
        10.7
        HT5VLAFXY_n01_dDAP_HALObZIP63_GSTbZIP53_Col_B_k
        9.7%
        42%
        0.0
        HT5VLAFXY_n01_dDAP_HALObZIP63_GSTbZIP53_Col_B_k-b
        9.7%
        42%
        0.0
        HT5VLAFXY_n01_undetermined
        79.2%
        45%
        23.4
        HT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k
        37.8%
        39%
        1.1
        HT5VLAFXY_n01_wg_HALOCCA1_pGSTLHY1_Col_B_k-b
        37.8%
        39%
        1.1
        HT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k
        41.1%
        39%
        3.6
        HT5VLAFXY_n01_wg_HALOLHY1_pGSTCCA1_Col_B_k-b
        41.1%
        39%
        3.6
        HT5VLAFXY_n01_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k
        27.7%
        39%
        8.9
        HT5VLAFXY_n01_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b
        27.7%
        39%
        8.9
        HT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k
        26.8%
        39%
        22.5
        HT5VLAFXY_n01_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b
        26.8%
        39%
        22.5
        HT5VLAFXY_n02_13YCol_14YC24_15YCol_16YC24_lib_k
        30.3%
        44%
        1.2
        HT5VLAFXY_n02_13YCol_14YC24_15YCol_16YC24_lib_k-b
        30.3%
        44%
        1.2
        HT5VLAFXY_n02_13YCol_14YC24_lib_k
        51.0%
        43%
        0.0
        HT5VLAFXY_n02_13YCol_14YC24_lib_k-b
        51.0%
        43%
        0.0
        HT5VLAFXY_n02_13YCol_14YCol_15YCol_16YCol_lib_k
        19.6%
        42%
        0.5
        HT5VLAFXY_n02_13YCol_14YCol_15YCol_16YCol_lib_k-b
        19.6%
        42%
        0.5
        HT5VLAFXY_n02_13YCol_14YCol_lib_k
        19.4%
        42%
        2.4
        HT5VLAFXY_n02_13YCol_14YCol_lib_k-b
        19.4%
        42%
        2.4
        HT5VLAFXY_n02_13YCol_Col_lib_k
        29.8%
        49%
        1.8
        HT5VLAFXY_n02_13YCol_Col_lib_k-b
        29.8%
        49%
        1.8
        HT5VLAFXY_n02_14YCol_Col_lib_k
        22.6%
        41%
        3.3
        HT5VLAFXY_n02_14YCol_Col_lib_k-b
        22.6%
        41%
        3.3
        HT5VLAFXY_n02_15YCol_Col_lib_k
        58.4%
        48%
        3.2
        HT5VLAFXY_n02_15YCol_Col_lib_k-b
        58.4%
        48%
        3.2
        HT5VLAFXY_n02_16YCol_Col_lib_k
        22.9%
        40%
        6.0
        HT5VLAFXY_n02_16YCol_Col_lib_k-b
        22.9%
        40%
        6.0
        HT5VLAFXY_n02_HALObZIP53_pGSTbZIP63_Col_B_k
        29.4%
        40%
        11.2
        HT5VLAFXY_n02_HALObZIP53_pGSTbZIP63_Col_B_k-b
        29.4%
        40%
        11.2
        HT5VLAFXY_n02_HALObZIP63_pGSTbZIP53_Col_B_k
        49.6%
        36%
        0.6
        HT5VLAFXY_n02_HALObZIP63_pGSTbZIP53_Col_B_k-b
        49.6%
        36%
        0.6
        HT5VLAFXY_n02_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k
        61.7%
        44%
        1.6
        HT5VLAFXY_n02_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b
        61.7%
        44%
        1.6
        HT5VLAFXY_n02_SPL9_13YCol_14YC24_Col_B_k
        56.8%
        44%
        1.3
        HT5VLAFXY_n02_SPL9_13YCol_14YC24_Col_B_k-b
        56.8%
        44%
        1.3
        HT5VLAFXY_n02_SPL9_13YCol_14YCol_15YCol_16YCol_B_k
        50.2%
        39%
        2.7
        HT5VLAFXY_n02_SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b
        50.2%
        39%
        2.7
        HT5VLAFXY_n02_SPL9_13YCol_14YCol_Col_B_k
        40.8%
        41%
        0.7
        HT5VLAFXY_n02_SPL9_13YCol_14YCol_Col_B_k-b
        40.8%
        41%
        0.7
        HT5VLAFXY_n02_SPL9_13YCol_Col_B_k
        67.4%
        45%
        0.6
        HT5VLAFXY_n02_SPL9_13YCol_Col_B_k-b
        67.4%
        45%
        0.6
        HT5VLAFXY_n02_SPL9_14YCol_Col_B_k
        58.4%
        38%
        1.8
        HT5VLAFXY_n02_SPL9_14YCol_Col_B_k-b
        58.4%
        38%
        1.8
        HT5VLAFXY_n02_SPL9_15YCol_Col_B_k
        71.7%
        40%
        1.7
        HT5VLAFXY_n02_SPL9_15YCol_Col_B_k-b
        71.7%
        40%
        1.7
        HT5VLAFXY_n02_SPL9_16YCol_Col_B_k
        62.4%
        37%
        2.6
        HT5VLAFXY_n02_SPL9_16YCol_Col_B_k-b
        62.4%
        37%
        2.6
        HT5VLAFXY_n02_bZIP53_Col_B_k
        28.5%
        40%
        11.2
        HT5VLAFXY_n02_bZIP53_Col_B_k-b
        28.5%
        40%
        11.2
        HT5VLAFXY_n02_bZIP63_Col_B_k
        46.3%
        36%
        2.5
        HT5VLAFXY_n02_bZIP63_Col_B_k-b
        46.3%
        36%
        2.5
        HT5VLAFXY_n02_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k
        28.3%
        39%
        17.0
        HT5VLAFXY_n02_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b
        28.3%
        39%
        17.0
        HT5VLAFXY_n02_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k
        39.7%
        36%
        0.9
        HT5VLAFXY_n02_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k-b
        39.7%
        36%
        0.9
        HT5VLAFXY_n02_dDAP_HALO_GST_Col_B_k
        3.5%
        46%
        0.0
        HT5VLAFXY_n02_dDAP_HALO_GST_Col_B_k-b
        26.4%
        39%
        0.9
        HT5VLAFXY_n02_dDAP_HALObZIP53_GSTbZIP63_Col_B_k
        33.3%
        39%
        10.7
        HT5VLAFXY_n02_dDAP_HALObZIP53_GSTbZIP63_Col_B_k-b
        33.3%
        39%
        10.7
        HT5VLAFXY_n02_dDAP_HALObZIP63_GSTbZIP53_Col_B_k
        5.9%
        45%
        0.0
        HT5VLAFXY_n02_dDAP_HALObZIP63_GSTbZIP53_Col_B_k-b
        5.9%
        45%
        0.0
        HT5VLAFXY_n02_undetermined
        75.2%
        45%
        23.4
        HT5VLAFXY_n02_wg_HALOCCA1_pGSTLHY1_Col_B_k
        30.8%
        40%
        1.1
        HT5VLAFXY_n02_wg_HALOCCA1_pGSTLHY1_Col_B_k-b
        30.8%
        40%
        1.1
        HT5VLAFXY_n02_wg_HALOLHY1_pGSTCCA1_Col_B_k
        35.4%
        40%
        3.6
        HT5VLAFXY_n02_wg_HALOLHY1_pGSTCCA1_Col_B_k-b
        35.4%
        40%
        3.6
        HT5VLAFXY_n02_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k
        23.6%
        39%
        8.9
        HT5VLAFXY_n02_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b
        23.6%
        39%
        8.9
        HT5VLAFXY_n02_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k
        23.7%
        40%
        22.5
        HT5VLAFXY_n02_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b
        23.7%
        40%
        22.5

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 30/30 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        23,352,500
        16.0
        wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b
        8,892,518
        6.1
        wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b
        22,457,444
        15.4
        wg_HALOCCA1_pGSTLHY1_Col_B_k-b
        1,134,356
        0.8
        wg_HALOLHY1_pGSTCCA1_Col_B_k-b
        3,632,428
        2.5
        bZIP63_Col_B_k-b
        2,486,631
        1.7
        bZIP53_Col_B_k-b
        11,206,507
        7.7
        dDAP_HALObZIP63_GSTbZIP53_Col_B_k-b
        700.0
        0.0
        dDAP_HALObZIP53_GSTbZIP63_Col_B_k-b
        10,684,175
        7.3
        HALObZIP63_pGSTbZIP53_Col_B_k-b
        644,674
        0.4
        HALObZIP53_pGSTbZIP63_Col_B_k-b
        11,247,735
        7.7
        dDAP_HALO_GST_Col_B_k-b
        895,045
        0.6
        dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k-b
        908,647
        0.6
        dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b
        17,001,198
        11.7
        SPL9_13YCol_Col_B_k-b
        578,265
        0.4
        SPL9_14YCol_Col_B_k-b
        1,809,284
        1.2
        SPL9_15YCol_Col_B_k-b
        1,693,857
        1.2
        SPL9_16YCol_Col_B_k-b
        2,649,120
        1.8
        SPL9_13YCol_14YCol_Col_B_k-b
        702,030
        0.5
        SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b
        2,708,341
        1.9
        SPL9_13YCol_14YC24_Col_B_k-b
        1,315,093
        0.9
        SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b
        1,568,567
        1.1
        13YCol_Col_lib_k-b
        1,760,927
        1.2
        14YCol_Col_lib_k-b
        3,344,582
        2.3
        15YCol_Col_lib_k-b
        3,150,236
        2.2
        16YCol_Col_lib_k-b
        6,000,310
        4.1
        13YCol_14YCol_lib_k-b
        2,421,772
        1.7
        13YCol_14YCol_15YCol_16YCol_lib_k-b
        482,558
        0.3
        13YCol_14YC24_lib_k-b
        9,035
        0.0
        13YCol_14YC24_15YCol_16YC24_lib_k-b
        1,153,860
        0.8

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGGGGAGATCTCG
        15601214.0
        66.8
        GGGGGGGGGGGGGGGG
        258562.0
        1.1
        GGGGGGGGAGGATAGG
        222783.0
        0.9
        GGGGGGGGGCCTCTAT
        217784.0
        0.9
        ATTACTCGGGGGGGGG
        210533.0
        0.9
        TCCGGAGAGGGGGGGG
        147511.0
        0.6
        GAGATTCCGGGGGGGG
        133383.0
        0.6
        ATTCAGAAGGGGGGGG
        125019.0
        0.5
        GGGGGGGGAGCTCTCG
        123653.0
        0.5
        GGGGGGGGTCAGAGCC
        101958.0
        0.4
        CGCTCATTGGGGGGGG
        99834.0
        0.4
        GGGGGGGGTAAGATTA
        92190.0
        0.4
        GGGGGGGGAGGCTATA
        75417.0
        0.3
        GGGGGGGGAGATATCG
        62507.0
        0.3
        GGGGGGGGAGATCTCT
        57794.0
        0.2
        GGGGGGGGACATCTCG
        54742.0
        0.2
        ATTACTCGAGCTATAG
        52529.0
        0.2
        ATTAGAAAGCCTCTAT
        42351.0
        0.2
        GGGGGGGGGTCAGTAC
        41112.0
        0.2
        NNNNNNNNGCCTCTAT
        34581.0
        0.1

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        169,250,136
        145,892,395
        16.0
        11.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Sequence Length Distribution

        All samples have sequences of a single length (76bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.