FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22457444
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4210151.874723588312187No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC501980.22352499242567408Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA81200.057.9309359
GGTCGCC85850.056.75017556
GCCGTAT91450.052.4714460
TGGTCGC92150.051.9234755
GTCGCCG101950.047.99306557
TCGCCGT100450.046.16726758
CCGTATC101300.045.74441561
TCGGTGG108200.044.9028651
CTCGGTG103050.044.19164350
GGTGGTC111600.043.62768653
GTGGTCG114150.043.1125654
CGTATCA113300.041.54678762
CGGTGGT122400.039.74967652
TCTCGGT133050.034.0431949
GATCTCG141300.031.9058347
ATCTCGG145200.031.41072848
TGTGCCG103800.027.474570
GTATCAT202300.022.92261163
AGATCTC226800.020.21727446
CATTAAA242000.019.99748667
Produced by FastQC (version 0.11.5)