FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_dDAP_HALOLHY1_GSTCCA1_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22457444
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4210151.874723588312187No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC501980.22352499242567408Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA81350.057.91047759
GGTCGCC85450.056.68833556
GCCGTAT91900.052.2145660
TGGTCGC92200.051.81908855
GTCGCCG101250.048.152357
TCGCCGT99650.046.5731858
CCGTATC102650.045.2111561
TCGGTGG108850.044.86017251
CTCGGTG102400.044.40413350
GGTGGTC112200.043.36342253
GTGGTCG114950.042.8124654
CGTATCA113700.041.55438262
CGGTGGT123200.039.52018752
TCTCGGT132550.034.1453149
GATCTCG143200.031.45829647
ATCTCGG146100.031.19351648
TGTGCCG104650.027.25155670
GTATCAT198550.023.30258863
AGATCTC228900.020.04719746
CATTAAA240100.020.02463367
Produced by FastQC (version 0.11.5)