FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8892518
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1223251.3755946291028032No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC187600.21096386872649567Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC32900.055.95527656
CGCCGTA32350.054.63466659
TGGTCGC35050.051.9270255
GCCGTAT36750.050.37927660
CCGTATC36350.047.2749161
GTCGCCG39650.045.81133357
TCGCCGT39550.044.6887858
TCGGTGG39150.044.6130151
CTCGGTG36800.041.75509350
GGTGGTC44600.041.20096653
GTGGTCG45000.041.14562254
CGTATCA42200.041.0522162
CGGTGGT46300.039.46139552
ATCTCGG49350.031.98727248
TCTCGGT49500.031.39607649
GATCTCG48300.031.1611947
GTATCAT72600.022.85009463
AGATCTC73600.020.9726646
TAGATCT88850.018.23810245
TCATTAA93950.018.21519966
Produced by FastQC (version 0.11.5)