FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_dDAP_HALOCCA1_GSTLHY1_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8892518
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1223251.3755946291028032No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC187600.21096386872649567Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC32950.055.8711156
CGCCGTA32350.054.52689459
TGGTCGC35250.051.5332155
GCCGTAT36700.050.2572960
CCGTATC36300.046.9544661
GTCGCCG40100.045.2103157
TCGGTGG39150.044.52419751
TCGCCGT40200.043.8794858
CTCGGTG36700.041.67867350
GGTGGTC44050.041.63625353
GTGGTCG44700.041.3437954
CGTATCA41850.041.06088662
CGGTGGT45850.039.69632352
ATCTCGG49050.031.96944448
TCTCGGT49250.031.34247849
GATCTCG48450.030.77632547
GTATCAT72350.022.68710563
AGATCTC73850.020.75986946
TCATTAA93800.018.16954266
TAGATCT88650.018.12146845
Produced by FastQC (version 0.11.5)