FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_HALOLHY1_pGSTCCA1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_HALOLHY1_pGSTCCA1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632428
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410181.1292171517233103No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC36900.10158494538639168Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA5700.049.1203359
GGTCGCC8000.041.56155456
TGGTCGC8200.037.56270655
GCCGTAT9100.037.30632860
CCGTATC8250.031.81701761
TCGGTGG10750.031.25983851
GTCGCCG10000.031.14857
TCGCCGT9400.030.53037658
GTGGTCG11400.030.08910454
GGTGGTC11300.028.80703453
CTCGGTG8450.026.51228750
CGTATCA10650.025.9614962
CGGTGGT14050.024.4142752
GGTGGCC4650.018.81677456
GTGGCCG4200.017.49887857
ATCTCGG14450.016.95603248
GATCTCG13500.016.59358647
TCTCGGT13400.015.67365949
ATCGGTG6350.015.43505950
GCCGGAT5250.015.33277160
Produced by FastQC (version 0.11.5)