FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_HALOLHY1_pGSTCCA1_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_HALOLHY1_pGSTCCA1_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632428
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410181.1292171517233103No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC36900.10158494538639168Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA5600.049.9971459
GGTCGCC8050.041.3031256
TGGTCGC8150.037.79289255
GCCGTAT9100.037.6906760
CCGTATC8200.032.01080361
TCGGTGG11100.030.58931251
GTCGCCG10200.030.53704557
GTGGTCG11350.030.22144754
TCGCCGT9500.030.20879758
GGTGGTC11350.028.67993253
CTCGGTG8500.026.76796750
CGTATCA10550.026.2073962
CGGTGGT14300.023.98728452
GGTGGCC4700.019.36112656
GTGGCCG4150.018.552957
ATCTCGG14550.017.07994348
GATCTCG13700.016.60671847
TCTCGGT13650.015.89937349
GCCGGAT5250.015.33266560
ATCGGTG6450.015.19564950
Produced by FastQC (version 0.11.5)