FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_wg_HALOCCA1_pGSTLHY1_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_wg_HALOCCA1_pGSTLHY1_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134356
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120931.066067442672318No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC25720.2267365800507072Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4050.054.44307359
GGTCGCC4450.052.69762856
GCCGTAT4300.051.27777560
CCGTATC4050.048.39384561
TGGTCGC5000.046.20494555
CGTATCA4700.042.44386762
GTGGTCG5550.042.2567854
GTCGCCG5450.041.7420757
TCGCCGT5300.041.60272658
TCGGTGG5550.039.73398651
CTCGGTG5250.038.00406650
GGTGGTC6100.037.2990853
CGGTGGT6150.034.7191752
TCTCGGT7000.028.50179549
GATCTCG7150.028.39214547
ATCTCGG7300.027.80874648
GTATCAT8450.023.60783263
AGATCTC9150.022.1862146
TCATTAA11750.017.57247566
TAGATCT12300.017.35728645
Produced by FastQC (version 0.11.5)