FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23352500
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5785172.4773236270206613No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1760940.7540691574777861No Hit
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT1039640.4451943046783Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT473080.2025821646504657Illumina Single End PCR Primer 1 (97% over 35bp)
GTGAGGTGACTGGAGTTCAGACACCTCACAGATCGGAAGAGCGTCGTGTA282880.12113478214323947TruSeq Adapter, Index 1 (95% over 22bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT264350.113199871534097Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT245150.067.0685351
ACTCTAT408950.064.9393541
GCATGCG108750.063.6843224
CTCTATA320600.063.208612
CGCATGC113950.063.0356333
AGGTGAC86450.060.8429074
TGAGGTA120300.060.1756942
CCGCATT44000.058.9524352
TATAGAG276100.058.7725455
TAGAGTA417250.058.2728587
ATAGAGT419700.057.8486866
TGAGGTC63600.057.6820072
GATCGTG61100.057.062962
GATCGTA114150.056.6711772
AGTAGAT428650.056.66385310
TCGCCGT343900.056.5737170
GATAGGG233950.056.04777548
GATCGTT63700.055.7230262
ATAGGGT230300.055.19031549
TAGGGTG236300.054.84049650
Produced by FastQC (version 0.11.5)