FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALObZIP53_GSTbZIP63_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALObZIP53_GSTbZIP63_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10684175
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGT8704.1736166E-77.24120162
CGCCCGC6804.3209395E-57.20685317
GCCCGCG11355.1326315E-65.859106561
CCCGCGA9609.807521E-55.83420119
CGGGCAG8457.2588166E-45.799326453
CGCGTCG15453.426794E-85.663238564
CCGCGTC16452.3010216E-85.531753563
AGCCCGC10951.1030278E-45.433885660
TGTAGCC64950.05.334942361
GAGCCCG11251.6561759E-45.28885859
CCCGCGC12058.258209E-55.227940665
CCGCGAC8800.007337245.17132120
AGAGCCG30950.05.08765670
GGTGTAG71700.05.07667959
GCCGCCC9000.0095468965.055834315
GTGTAGC69850.05.010825660
Produced by FastQC (version 0.11.5)