FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALO_GST_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALO_GST_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74.046242774566474No Hit
TAAAAGCAAGACGTGTAGGCGGGTCAACTCATCAAGTTCCCATTGAAATA10.5780346820809248No Hit
CTCGTGAACTTAGCCGATACAAAGCTATATGATAGCACCCAATTTTTCCG10.5780346820809248No Hit
AACGCATAACATCGGATATACTCCCGTCGCAGCATTCTTAGAGAGAATCG10.5780346820809248No Hit
ACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGG10.5780346820809248No Hit
TTAAGCGCCATCCATTTACGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA10.5780346820809248No Hit
ATCTTCGTAATTCTTGTAGTGATTCATGATGAATCTGTTGTCACCATTAC10.5780346820809248No Hit
TGTAACAACACCCTTTTACACAAACAAAACAGAAGATCAAATTACCAACC10.5780346820809248No Hit
CAAGGCATCTTCAAAGTGAATCGTATTCCATAAATATGATATCTATCTAG10.5780346820809248No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTA10.5780346820809248No Hit
TTTTCGATCAATACCTAAAAGCTTGGAGCGTGCCACTGCTCAAAAACTAA10.5780346820809248No Hit
ACCGCATAGTTTGGTTACCAAATCTAAGTGCCACAGCAACAGAGACCCTC10.5780346820809248No Hit
GTGAGGTTTATTAGAATTTTATAAAGGTAGATTTTTTCTTTTTGTAGTTA10.5780346820809248No Hit
ACCGCATAAAAGCAAAAGAGGGAGAGGAAGGCCAAGAAAGAAAGGGCTTC10.5780346820809248No Hit
GCCGCATGGGGTTGATCGGTAGGGCGTCGGGTAGGGAAAGTGTCTACGGC10.5780346820809248No Hit
ACCGCATATGGTGGTGGTGGTCTCACTGATAGTGATTTCGGTATATGTTC10.5780346820809248No Hit
TTTCCCTTCGGCCACGCGCTCATGGATCGTCGTCAGCTCATTCAAGCACT10.5780346820809248No Hit
ACCGCATCAAGGACCACCTTGCAAGGCTAAATACTCCTGGGTGACCGATA10.5780346820809248No Hit
AGCGCATTGATATGTTTGGTGGATCTATCAATGAATATTTGCAATTTTTG10.5780346820809248No Hit
TTCTAAAGTCGAATATGACTTGATGTCATGTGTATGATTGAGTATAACAA10.5780346820809248No Hit
CTTGCTCTGTTTCCAAGGCAGGCTTCCTTAACTTGCAACCTTTGTGCCTT10.5780346820809248No Hit
ACCGCATTTTCGCATCGTAACTACCTAAGTCGGTGGAAAACACAACGTTT10.5780346820809248No Hit
TTGCGGTTTAAGTTCTTATACTCAATCATACACATGACATCAAGTCATAT10.5780346820809248No Hit
AACGCATAAGGGATAATTGAAGACAGAAAGTCAAATCTCAACACAAAGAA10.5780346820809248No Hit
GTGAGGTAGATATGCTCGTTAACACCAGCGTTGAACCCAACACTTGCTTT10.5780346820809248No Hit
AACACTACTTAGGCTTTTAGGATAACCTTGCGGTTTAAGTTCTTATACCC10.5780346820809248No Hit
AACGTATGATTCGTCGTTTCTATTGGGTCAAGATTTTCATATACTGTTCA10.5780346820809248No Hit
TTACCTCTCAGTTACTTGTCTCGGTGACATTGAATATGTGATATTGTTAT10.5780346820809248No Hit
GTGAGGTATCAAAACTATGTTGGGTAACTGAATAAATTTCTAACATATGT10.5780346820809248No Hit
ACCGCATTATTTCCCGCCCAATTTTTGTTTTCAAAAATATTTTTCACGCC10.5780346820809248No Hit
ACCGCATTATAAATTTAAAACGAGGTGGTTTTGTTGGTATCCAATTTGTA10.5780346820809248No Hit
TTCTTTCCTATTCAACTCCGAGAGTTTGATCGTTGTCTTACTCTCTACTG10.5780346820809248No Hit
TACAGGGACAGAAGCTTTGAGAGATAGTAGTATGTGTCTATGTGACATAA10.5780346820809248No Hit
AGTCAACATACAAGGACCCGCACCAGTATAACGAACTGTACACAAAGCGT10.5780346820809248No Hit
TTTTAGTTTTGAATGAAGCAAAAGTAAGTCGATAATAATCTAAAAACTAG10.5780346820809248No Hit
CTGAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCGTA10.5780346820809248No Hit
AGTCATATTCGACTCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAA10.5780346820809248No Hit
AGAGTATATGCGTTATCTTCAAAGTTCACTTGGAGATTCAGGACCCAATG10.5780346820809248No Hit
TTTTCAATGGCAAATCAAGGCATATTCGAAGGAGACATGCTACAATACGA10.5780346820809248No Hit
CACACACACAAATACAAATCCATTATAGATGCAAAAGTGTAAGAAAAGGT10.5780346820809248No Hit
AAAACGGACTATGTAATGTACTTGAATCTGCCGGGTTACGGGGGGCTTTT10.5780346820809248No Hit
GTGAGGTTGAGACGCTTGTATAAAGGAATGTCCGGACTGGTATGGAAAGC10.5780346820809248No Hit
GTGAGGTGTCCAGGATTCCTCCACCAGGACTTGAAATCGTCGAGGGAAAA10.5780346820809248No Hit
GAAAAATGCAGTTACATCTTCGATTTCTATCAAGTTCTTGATCAAGATCT10.5780346820809248No Hit
ACAATCAATAGAAAATAAGAGAGAACAATAACGAAAAAAAAAAAGAAAAC10.5780346820809248No Hit
GTGAGCTTGTTTCTTCAGTTGCAACAATCATTTGTAGGCGGAGAAGATGC10.5780346820809248No Hit
ATGCGGGGGGGTGCGGGGGGCAGACGGGGCGTCGCGGTGGGCCAGCGCTG10.5780346820809248No Hit
AAGAAAAAAAGAGTTTCATCACTATAAAATATATTCACTTAAGTTGATTT10.5780346820809248No Hit
TCTTCTTTTTCTATGACACCCAAAGGGACTCCCTTCAGGGATGAACTTCA10.5780346820809248No Hit
GAATGAGACAACGTGAGAGAAACTTATCATTGTCGCAACGGTAGGGGACT10.5780346820809248No Hit
ATTTGAATATACAAAAAACATTACACGTGTTCTTTATAAAGCCATCTTGA10.5780346820809248No Hit
ATCATCCTTTCAAGCCCTAGAAGAACGGCTGCAGGAAGCTCAAAGGAAAA10.5780346820809248No Hit
CAATAAAAAGCTCTAACTTAGGAACATTACAACGAACTAATATTTAATAA10.5780346820809248No Hit
TAATAAACAAACATTTTCAAAAATGTAATAAATGAAGCGTCAGAGTTATT10.5780346820809248No Hit
GGAATTGAGAAGTTTGTTTCAAAATGTTAATAGAGCTTCCTAAATATCTT10.5780346820809248No Hit
ACATTGTGGTCAATCACGTAAGATTGGTGAATTTGTTGAAAGTACGGAAA10.5780346820809248No Hit
GTAGATTTATGACAAAAACATTTGAAGATATATTCAAATTGATTGGATTT10.5780346820809248No Hit
TTTACCTTTCTTTTTTGTTATAGAGCTCTCGTTTCCCTGAGCTTGAGGTC10.5780346820809248No Hit
CATTTTCGCCACGTGTACTACAAAGATCAAAGCAGAGACCTTTCTGATCA10.5780346820809248No Hit
TTTCTATTGATAGATAATAACGAATTTCTTCATTTCCACGTCATAATAAT10.5780346820809248No Hit
CGCGGGGGGGGGGGGGGGGGGGCGGGGCGGGGGGGGGGGGGGGGTGGGGG10.5780346820809248No Hit
CCTTGGACGGTTTTAGTGTTAAGTGTTCTATATTTTCAAGATTTAAAATC10.5780346820809248No Hit
ACCGCATAGCAGCTAGATGACTCGATCTCCGCCATGCTCGCAGACGCACA10.5780346820809248No Hit
GTGAGGTCACTTACCCATTCAGTGACTTTGGCACTGGATGGACGTTACCA10.5780346820809248No Hit
AACATAATAAGAGGCATCATCAGGATGTAAATCAAGAATCTTCTTAATCA10.5780346820809248No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.5780346820809248No Hit
ACCGCATAGTCTTGGCATTTAGAAATCTCTCTCTTCTTGAGTCATTGTAA10.5780346820809248No Hit
ATGACTACGTACAATCCCCACCGACCTCGCCTTACTCAGCCTCATCGCTG10.5780346820809248No Hit
ACGCAAATTAATTATATAGAAATTTCTTCCTGTAGCCAAAAAAATTAAGT10.5780346820809248No Hit
GTGAGGTCTTTATAAACCAAGTTGAAGCTTGATATTATTTGTTTTGCCAA10.5780346820809248No Hit
GTCTTGAGTCCTATACCTAAATGGTGTGTTGTGTGATGCAGTGTATGCCA10.5780346820809248No Hit
ACGAGTAATATGAAATGTTAAATAACAAATAATCATAAAACCTCACACTT10.5780346820809248No Hit
GTGAGGTAGAAAAGAAATGATTCTAACAAATTGTAAACTACATAATGATA10.5780346820809248No Hit
AGCTTGTCCGAGACATCAAAATCACGAATAGGTATGACATTGCTAATGAG10.5780346820809248No Hit
GTGAGGTGACTGGAGTTCAGACACCTCACAGATCGGAAGAGCGTCGTGTA10.5780346820809248TruSeq Adapter, Index 1 (95% over 22bp)
CGTACCTCCTGCTCTCTTCACCTCTCTCTTGATATGTAATAATAATCAGA10.5780346820809248No Hit
ACCGCATAGAACGACGGGACGAGCGTGAACGCTAAATTCAATGAAAGAGA10.5780346820809248No Hit
CTGTACCGCTGGACTCACTACCACCACCTGCCTCTGAAGGTCCTCCTCTA10.5780346820809248No Hit
GGGGGGGGCTGCCGGGGGGGATGAGGGCGATGTAAGAGATTGAGCGAGCT10.5780346820809248No Hit
ACCGCATAGAAAGCTACATTTTGCACATATATTCCTTCACAATCAGTTCT10.5780346820809248No Hit
AAATCACGACCGACGGGAGTGAATGACGGCGAGGCGGCGATCAATGGAGG10.5780346820809248No Hit
ACCGCATCCCGGAAAACCGGAAGCAAACCCAATGGAACATATTACATAAT10.5780346820809248No Hit
CCGGGGGGGGGGGGGGGGTGGGGGTGGCGGGGGGTGCGGGGGGGGCGTGG10.5780346820809248No Hit
ACCGCATAACCACACAGCGTTTTATATCAGACACTATTCTGGATCTTTGC10.5780346820809248No Hit
AGTCGGTCAAGGTACAGCTGCGGGTCAAGCTGTCGAAGGTATCGCGAGAC10.5780346820809248No Hit
TGTGGGGGGGGGGGTGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGG10.5780346820809248No Hit
ACCGCATTATGTGTAGTGAGCAGCGGTGTTAGTTAGACAGCTGAGTTTGT10.5780346820809248No Hit
GAAATTGTGGCAACGCCATCGCCGCAATTCATGCTTTACTGAGCTGTTCA10.5780346820809248No Hit
CCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCT10.5780346820809248No Hit
TGTTCACGTAATTGAAATGTAGAAAACGAGTTTTGAGTAAGATTTAACAG10.5780346820809248No Hit
GTCTGATTGTGTTTCGTCCGGTTTATTTCGGATTCCTAAAGAAGAAGGGA10.5780346820809248No Hit
GTGGGGTGTGTTGTCGGGTGGGGGGGGGTGGTCTGGGGGAGAGTGGCTTG10.5780346820809248No Hit
ACCGCATCTTACCAGAAAAAATAATTCAAATAATAAACTAGAATTAATGT10.5780346820809248No Hit
GGGCGCGGCTGGCGGCGCGGGGGGGGGGGGGCCGCTGCCGCGGTGGGGTG10.5780346820809248No Hit
CAAGCGTCATTAACCTATTTCCTAATTCTAAGATATCTTCAAATCTTCTT10.5780346820809248No Hit
GTTCCGAACCTAACTCCTTTCCTCACTAAGGGTGATTCATCTTACACGTG10.5780346820809248No Hit
ACACACTCTATTAGTTATCATTAACCATCTCACTAATTAAACCTTAATGT10.5780346820809248No Hit
TAATACAAACTCGTAGGAAGCAAAAATAGACCATTCACTCCGAAAAGGAC10.5780346820809248No Hit
GAGTATAACAACTCAAACCGCAACCCGATCCTAAAAGCCTAAGTAGTCTT10.5780346820809248No Hit
TCTTGCTCACATTGATTAGTGGTAACTTGCAAATTTAATGTCTATAATTT10.5780346820809248No Hit
CGCGAGAGAGGCGCTCGGGGGGGGGGCGGGGGGGGGGCGGGGGGGGCGGA10.5780346820809248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGG10.5780346820809248No Hit
ATATAACTTGTTCGCATGATATTACCGAGATGTCCCCACGGGCATCTTTT10.5780346820809248No Hit
ACCGCATCATCAAGTCATATTCTACTCCAAAACACTAACCAACCTTCTTC10.5780346820809248No Hit
AGTTTCCCGTTAGATCAGTCGCCGTTTTGTTAATAAAAGGATTAGATCTT10.5780346820809248No Hit
TCTATACACGTCTTTTTTTAGGGGGTCTACAACCATTATGTGGCATAGGG10.5780346820809248No Hit
TCAGACAAGGAGATCCCATGTCTTCTCATATATTTGTGCATATCATGAAT10.5780346820809248No Hit
AAACAATATAATCCAAACAAAGATATTCCTGAACCCAAAAACAGTATTAA10.5780346820809248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG10.5780346820809248No Hit
TCCACCAACAGATGTGTAACAAAATTGGGGTTATCCGCGAAAAACTATGG10.5780346820809248No Hit
CTTACGAAGCTCATATCTCTAGGGCAGAGGAGGCGAACCAAGTGTCTCAG10.5780346820809248No Hit
CCGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAGGATACAAAGCCA10.5780346820809248No Hit
AACGCGTGGGGTAGAGCGGAAGAGCGTCGTGTGGGGAAAGAGTGGACGTG10.5780346820809248No Hit
AAGGAGCCGGGTACACTGCATGTACACGTAAGTTGTTCACACATTTCACA10.5780346820809248No Hit
TAGTAATGATAGAAGGAAAAAATTATTTAAATTTGAGAACTAAACATATT10.5780346820809248No Hit
TAATACACGCATGACAGGAACTTATGTTCTTAAAAGCTTTTCATGACGAT10.5780346820809248No Hit
GTGAGGTAGAATATATACGATTGCATTGCATATACTAACGGATGCGATCA10.5780346820809248No Hit
ACCGCCTATTAATTTATGAATACTACATGCATGATTCAGTTGCGAAGACT10.5780346820809248No Hit
GTATGTATGATGACTGGACTTATGTCTGACCAAGGACTAGTTTGAGCCAT10.5780346820809248No Hit
GAAGAATGGGCGTGTCATGCGTGGGCTGACATGGATTCTTCGAGGCCTAG10.5780346820809248No Hit
AAAAAGATGTTTCAAATGCCTACTTTTGCTTGCAATATCCTTTAAGCTCT10.5780346820809248No Hit
AATACTTACTAGTTACTGCTATCTATAAAATATGAGAAAAGTAAAATAAA10.5780346820809248No Hit
GTGAGGTATTTGATTTTATAATCAGCAAGGCCAAGGAGATTTCAAGAATT10.5780346820809248No Hit
GCCGCAGGGGGGTGGGGGGGGGCGTGCTGGGGGGGGGGGACTGTGGGGGG10.5780346820809248No Hit
GCTTATGAAAATGTCGTACCACAAAGGAAAGGGTACAGCAACAATAACAC10.5780346820809248No Hit
TCAGAGAAAATGCCACCTCTAGTTGAATTAGTCTCTTATATAACACTTTT10.5780346820809248No Hit
ATATGGAGTGAAGGATCTAGTATCTTCTGTGACATCGTCCTTTTAGAGTA10.5780346820809248No Hit
AACATAGTTTCAGAATGCTTTTTAGAGTCTTTCTAGTCTTTTTCGTATGA10.5780346820809248No Hit
TCTTGATTTCCATGAACATTATCTAACTTAGCTACTGCTATCAATGAATT10.5780346820809248No Hit
ACCGCGTCTTTGACGGATGTGGGTTCTCTAGAGCCGTGATTCAGCTGGTG10.5780346820809248No Hit
GTGAGGGCTTCATTAAATATAGGAGAAAGTTTTGTGCCGAATTCAAAAAT10.5780346820809248No Hit
GCACCATTGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA10.5780346820809248Illumina Single End PCR Primer 1 (97% over 35bp)
TGTGAGTGTCGCCCATGGGCATCGACACCTTGCGGCTAGGAACTGGAACG10.5780346820809248No Hit
TCCGCATAGACAAGGAGAGACAGGTAACAGATCAGTTTTTATCCAAGCTA10.5780346820809248No Hit
GGGACGGTGATGAAACAGGTGGGGTGAGCGGGAGTGGGGGGGAGGGGGGG10.5780346820809248No Hit
ACGGGTCTAGAGGAGCGCGGAAGAGCGGGGGGGTGGGGGGGGGGGAGGGG10.5780346820809248No Hit
GTATCTTAAAGGTAAATAAGATACAGCACAGAGACAAAGTCGGTTGTAGC10.5780346820809248No Hit
GTGAGGTTATTGCTTCTTCCCCACCAATAATCCCAACTCCTTCAACCCGT10.5780346820809248No Hit
GTGAGGTGTAGAGTTTGCAACAAAATAATAAAATAGTAAATACCACAAAT10.5780346820809248No Hit
ACCGCATAAGACATATCAAGTAGCTGACCTACTTTGTGATGTACTTTCTG10.5780346820809248No Hit
GTGAGGTAACAAGGCGAATCTTTACTTGATTATGATCACCATAACATCTG10.5780346820809248No Hit
GTATGAAGTGGGGTCGAGCGCGCGGGGGGGGGCGTGTGGTGGGCCGGGGG10.5780346820809248No Hit
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGT10.5780346820809248Illumina Single End PCR Primer 1 (97% over 34bp)
AGCTATTCATCTCTATTCTCAGAGATTCATCCAGTTTTCAATATAGTGAA10.5780346820809248No Hit
GTGAGGTATCTGCAGTGGTGTTTTCATTAAATCTTTTTTTGAATGGATTT10.5780346820809248No Hit
TAGGATTTTGGTTATAAAGTTTGGGGACTATGGTTTTGGGTTTAGGTTTA10.5780346820809248No Hit
ACTCGAGCTCGTAGATGAGATTTCGATGTTTTTTAGAAATCGAGTATGAA10.5780346820809248No Hit
GTTTTCGTTTTTTTTCTTTTCTATTAAAATATTTGTTGAGTCTTGTTCCT10.5780346820809248No Hit
TGCATGGTCAATGACTCATGATCCTTGTTCTTTATGACAATATTATTCTG10.5780346820809248No Hit
ACAAAGATGTTTCAAATGCCTACTTTTGCTTGCAATATCCGTTACGCGCT10.5780346820809248No Hit
TATAAAACCGTATATTTCTCTAAATACTTAGTCTTTTATATAACATATCA10.5780346820809248No Hit
CAGATCTGTAATCTGTCTTCATCATCCCTTGGGTTTTCATTTTTTCACAC10.5780346820809248No Hit
GGCCGAGGAAGCCATTAGAGGAAAGAAACAAGCCCTCTAATCTTTTGAGA10.5780346820809248No Hit
GTGAGGTAGTGAAGTGGAGGGGCTTTAAGGTAATTACGGCTGCTGCTGAA10.5780346820809248No Hit
AGTCATAAAGGGTAGACCTTTCACTGAAACTCTTGTGGAGATTCTCTACT10.5780346820809248No Hit
ACCGCATAGGTGAGCTAGCCCATGTTACAAGAGCTAAATACTAATCCGAT10.5780346820809248No Hit
TACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCCCTAT10.5780346820809248No Hit
GCCACATAACAGATCGGAACACCGTCGTGGAGAGGAAGAGGGTACGTCCA10.5780346820809248No Hit
GTTCTATAGGCAATGTCCTCTACTCCTGAGAGATCACAGGAACTGTTACC10.5780346820809248No Hit
CAGAAATGAAATTAAAAAATCATGTTCAGATGAAAGAAAAAAAAAAGTAC10.5780346820809248No Hit
GTGAGGTAAGGTGCGAGGAACTCTCGGCTTATCCTGTTTTAAAGATTTAA10.5780346820809248No Hit
CCTACATTCCTTGTGGATAAACAGAAAGCCAATTTAATCCAGTCTGCATC10.5780346820809248No Hit
AGTTTTTGTGATCTTTGGCAGATGCTGACATGACTAAGGATGACAAGCTC10.5780346820809248No Hit
ACCGCATATAAAAACATTCCTCCTAGAGTAGCTGTTAATATGAATAACAG10.5780346820809248No Hit
GGATAGGGGTAAAAGAAAAAGAGATTTTCGCCGTTTATGGATCACTCGAA10.5780346820809248No Hit
AAAGCTTTCATGGTGTAGCCAAAGTCCATATGAGTCTTTCGCTTTGTGTC10.5780346820809248No Hit
GTGATGTTTAGTCATTAAAAATACACTTAAATCAAGATTGAATAAATTAA10.5780346820809248No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers