FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALO_GST_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALO_GST_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895045
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17730.19809059879670857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1508.8039087E-1025.67072559
GGTCGCC1454.59926E-721.72757556
TGGTCGC1558.731513E-720.32579455
CTCGGTG1651.5890855E-619.09499550
GAGCCCG1000.0097017617.49591838
TCGCCGT2401.9553954E-716.04420558
GTCGCCG2452.4716792E-715.71677157
GCCGTAT2505.2515607E-614.002213560
AGCCTCA1600.00908786613.12634261
TCGGTGG2254.738449E-412.44710951
CCGTATC2601.1746872E-412.11662361
CGGTGGT3054.0580206E-511.47658352
CGTATCA3054.0649757E-511.47465862
GGTGGTC2802.3407793E-411.25115153
GATCTCG2600.001592774210.77093447
GTGGTCG3004.4296664E-410.5016654
ATAGGGT6201.4122634E-89.59470
GATAGGG7001.0513759E-99.49725470
CTATACA3550.00207707688.871175
GGATAGG7503.6488927E-98.86459869
Produced by FastQC (version 0.11.5)