FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908647
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14580.16045835181319038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1451.7289494E-824.1370776
GGACTGT1709.318683E-820.5875077
ACTGTAG2352.7720725E-614.892279
GACTGTA2253.0342908E-513.99873358
TGGCGAT2153.2371478E-413.022078568
GCGGAAA2250.006697802310.88910363
CTGTAGA2903.246E-410.86108610
ACGGACT2600.001595387410.7688495
GCTATAG4804.2854698E-48.01966767
TGTAGGC8101.2552664E-77.777074366
GTAGGCT6805.200911E-67.71946567
TATAGTG5006.634091E-47.69930369
AGGCTAT6452.14421E-57.59621269
TAGGCTA6552.6299826E-57.480239468
GGCTATA6854.7542897E-57.152637570
AGATGGA5550.00200796246.93707359
CTATAGT6652.5616933E-46.841486568
GAGTGTA10401.6090962E-76.730901263
AGTGTAG10752.933084E-76.51175664
ATAGTGT5950.0041745496.470002770
Produced by FastQC (version 0.11.5)