FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALOCOLSPL9_GSTC24SPL9_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908647
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14580.16045835181319038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1401.1907105E-824.9991156
GGACTGT1709.318683E-820.5875077
ACTGTAG2302.2167842E-615.2160159
GACTGTA2253.0342908E-513.99873358
TGGCGAT2153.2371478E-413.022078568
GCGGAAA2200.005666635511.13658363
CTGTAGA2852.7632355E-411.05163210
ACGGACT2550.001356780910.9800035
GCTATAG4804.2854698E-48.01966767
TGTAGGC8151.3920726E-77.72936266
TATAGTG5006.634091E-47.69930369
GTAGGCT6855.7702073E-67.663118467
AGGCTAT6502.3757593E-57.537779369
TAGGCTA6602.9088655E-57.423570668
GGCTATA6854.7542897E-57.152637570
AGATGGA5450.00165705497.064358759
CTATAGT6702.8076E-46.7904368
GAGTGTA10451.7556886E-76.698696663
AGTGTAG10803.1898526E-76.48160964
ATAGTGT5950.0041745496.470002770
Produced by FastQC (version 0.11.5)