FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17001199
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285670.16802932546110425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG41550.020.71762870
CTATGTG137200.010.02338570
GGTCGCC17250.09.535809556
GCCTCTA195900.09.37782469
TGGTCGC18850.09.28406455
TGCCTCG38850.08.73688570
CCTCTAT210050.08.71274470
CTCTATG180750.08.53760870
TGTGTAG104800.08.21390770
GTGCCTC268300.08.17756769
GTGTGCC353700.07.696982470
TGCCTCT241400.07.624729670
TGTGCCT307600.07.30347968
GTGTAGA92700.07.285402370
TCTATGT196150.06.903985569
AGTGTGC404400.06.775289569
GAGTGTG496200.06.607828670
TATGTGT195750.06.16727570
GTGCCTG27250.06.163830370
TCGTGTT87500.06.158694370
Produced by FastQC (version 0.11.5)