FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_dDAP_HALOC24SPL9_GSTCOLSPL9_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17001198
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285670.16802933534448572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG43350.019.85721670
CTATGTG136050.010.51954870
GGTCGCC17250.09.53598256
GCCTCTA190250.09.06772769
TGGTCGC19500.08.79523755
CCTCTAT202600.08.60126370
TGCCTCG37100.08.583014570
CTCTATG175900.07.89763470
GTGTGCC347200.07.82085770
GTGCCTC257950.07.610262470
TGCCTCT233600.07.519812669
TGTGTAG104850.07.342206570
TGTGCCT301050.07.311191669
TCTATGT196150.07.118045369
GCGTCGG25700.07.080151670
AGTGTGC398900.06.833600569
GTGCCTG27200.06.68970270
GTGTAGA95050.06.66344470
GAGTGTG488300.06.607186370
GTAGGGG27200.06.303757770
Produced by FastQC (version 0.11.5)