FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_bZIP63_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_bZIP63_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486631
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49890.20063290452021226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2702.419256E-916.8508959
GGTCGCC3005.5661076E-1016.33174156
GCCGTAT3251.7262209E-915.07666860
TGGTCGC3454.0035957E-914.20380155
GCTAGCG1500.00593371814.00145251
CGGACTG3507.405106E-812.9984726
GGACTGT4306.6520442E-912.2078677
TCGGTGG4702.4723704E-811.17137151
CCGTATC4156.7574E-710.9636761
CAGTCCG2953.8006838E-410.6788627
GTAGGCG2300.007903472510.65027970
CTCGGTG4955.298716E-810.60716150
GGTGGTC5451.9696017E-810.27588253
CGTATCA5159.4829375E-810.19400262
ACAGTCC3156.9519907E-410.0014426
CGGTGGT7051.1459633E-109.929708552
GTGGTCG5452.162833E-79.63363954
GCACCTC3050.00599375659.17924911
GTCGCCG5452.2412205E-68.99012957
CTGACGT4200.00101814228.3338632
Produced by FastQC (version 0.11.5)