FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_bZIP63_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_bZIP63_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486631
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49890.20063290452021226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2551.1314114E-917.84211259
GGTCGCC3057.021299E-1016.06400356
GCCGTAT3201.3860699E-915.31223660
TGGTCGC3403.259629E-914.41267755
CGGACTG3558.912684E-812.8153926
GGACTGT4357.896233E-912.0675437
TCGGTGG4652.1114829E-811.29149151
CCGTATC4156.7574183E-710.96366661
GTAGGCG2300.00790348810.65027670
CTCGGTG4955.298716E-810.60715850
CAGTCCG3004.438691E-410.50087527
CGTATCA5108.22165E-810.29394162
GGTGGTC5502.269735E-810.18246353
ACAGTCC3156.9520035E-410.00143726
CGGTGGT7101.3096724E-109.85977852
GTGGTCG5452.162833E-79.63363654
GCACCTC3050.0059937729.17924611
GTCGCCG5452.2412278E-68.99012857
CTGACGT4159.0414E-48.4342682
TCGCCGT5703.8127753E-57.98199758
Produced by FastQC (version 0.11.5)