FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_bZIP53_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_bZIP53_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206507
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134070.11963585084986784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG20100.09.92309470
GTGTGCC73300.06.635583470
GCGTCGG26500.06.60225770
GTAGGGG20800.06.56099370
GCCTCTA52000.06.39281969
GTGCCTC62000.05.813232469
AGTGTGC85200.05.749904669
CCTCTAT57350.05.67437270
TGTGCCT73350.05.62929669
AGAGCGG29950.05.491225770
TGCCTCT65350.05.140502568
GGTCGCC14451.0073552E-55.08640456
GAGTGTG137850.05.076817570
AGAGTGT140650.05.050431369
Produced by FastQC (version 0.11.5)