FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_bZIP53_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_bZIP53_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206507
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134070.11963585084986784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG19950.09.12068270
GCGTCGG26350.06.639818770
GTAGGGG21050.06.483049470
GTGTGCC71200.06.29066970
GCCTCTA51300.06.07076469
GTGCCTC60700.05.76477169
AGTGTGC82300.05.48479869
CCTCTAT56600.05.440428770
AGAGCGG29650.05.428752470
TGTGCCT72250.05.32752669
CGGAAGC44150.05.072430670
AAGAGTG162500.05.06035970
GAGTGTG134300.05.028612670
Produced by FastQC (version 0.11.5)