FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_16YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_16YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649120
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72650.27424201244186747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT2504250.069.903991
GATCGTA1022000.069.634792
GATCGTC486050.069.237532
GATCGTT564150.069.2263262
GATCGTG456700.069.203352
ATCGTAA378500.068.581333
ATCGTAG187100.068.359173
ATCGTAT352600.068.317893
ATCGTAC130000.067.932243
ATCGTGT164650.067.5607153
ATCGTGC54150.067.3552253
ATCGTGA165500.067.340623
ATCGTCA181300.067.2832643
ATCGTCT179000.067.228723
ATCGTGG89050.066.743013
ATCGTTG133550.066.624463
ATCGTTA163550.066.217353
ATCGTTT210400.065.962873
TCGTAAA157400.065.750564
ATCGTTC87500.065.645143
Produced by FastQC (version 0.11.5)