FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_16YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_16YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649120
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72650.27424201244186747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT2504250.069.90261
GATCGTA1021650.069.641522
GATCGTC485850.069.244432
GATCGTT564450.069.214342
GATCGTG456900.069.20372
ATCGTAA378000.068.58873
ATCGTAG187500.068.3813553
ATCGTAT352750.068.328523
ATCGTAC129600.067.952853
ATCGTGT165000.067.5871053
ATCGTGC54100.067.417473
ATCGTGA165750.067.365753
ATCGTCA181750.067.309263
ATCGTCT178400.067.258633
ATCGTGG88450.066.760453
ATCGTTG133950.066.582293
ATCGTTA163600.066.23993
ATCGTTT210500.065.9315263
TCGTAAA157100.065.764724
ATCGTCC83550.065.648473
Produced by FastQC (version 0.11.5)