FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_15YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_15YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693857
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT1523548.994501897149524Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT647623.823345182031305Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG408622.412364207840449No Hit
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA126550.7471114739910157Illumina Single End PCR Primer 1 (97% over 35bp)
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA82900.489415576403439Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAT54620.3224593339343286Illumina Single End PCR Primer 1 (97% over 34bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA48430.28591551707139384Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGGAT41330.24399934587158184Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG35230.20798686075625036Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA30310.178940725220606Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGT29250.17268281797105658Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAT20950.12368222346986787Illumina Single End PCR Primer 1 (97% over 34bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGGAT19840.11712913191609445Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTTTAGGAT18240.10768323418092554Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAA16960.10012651599279042Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT1440350.069.659911
CTCTATG206150.068.292292
CTCTATC242450.068.202552
CTCTATA687350.068.1637342
CTCTATT336750.068.094642
CTCAATA92400.065.158252
TCTATAA168150.065.136083
TCTATAC69000.064.984983
TCTATAG315050.064.307553
TCTATAT178200.064.016163
TCTATCA89550.063.8312763
TCTATTG75000.063.426553
TCAATAG92950.063.416993
TCTATGA79600.063.323133
TCTATGG44050.062.6966063
TCTATCC44800.062.662733
TCTATGT74250.062.511513
TCTATGC27300.062.4423523
CTATAGA271250.062.2940144
TCTATTA102900.061.9791953
Produced by FastQC (version 0.11.5)