FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_14YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_14YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809284
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126580.699613769866975No Hit
GTGAGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG23350.12905657707689894Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1656900.069.861961
TGAGGTA750000.069.505032
TGAGGTC349550.069.213332
TGAGGTG268600.068.8576352
GAGGTAA292000.068.765923
GAGGTAT254450.068.6387563
TGAGGTT309550.068.376132
GAGGTAG137950.068.3513643
GAGGTCT132800.068.340123
GAGGTCA126950.067.629493
GAGGTAC81850.067.434843
GAGGTCC56400.066.9597243
GAGGTGT103150.066.9127663
GAGGTTA109550.066.518073
GAGGTGC26550.065.650213
GAGGTTG82550.065.591043
GAGGTCG45150.065.426853
GAGGTGA99800.065.301093
AGGTAAG62950.064.3760154
AGGTAGA60350.064.3660664
Produced by FastQC (version 0.11.5)