FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_14YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_14YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809284
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126580.699613769866975No Hit
GTGAGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG23350.12905657707689894Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT1656950.069.8640751
TGAGGTA749300.069.504572
TGAGGTC349250.069.222682
TGAGGTG268600.068.870672
GAGGTAA290700.068.7724463
GAGGTAT255050.068.641963
GAGGTAG137850.068.375563
TGAGGTT310600.068.370362
GAGGTCT132550.068.3105853
GAGGTCA127000.067.6583
GAGGTAC81650.067.428553
GAGGTCC56350.067.019143
GAGGTGT103200.066.948183
GAGGTTA109800.066.4941253
GAGGTTG82750.065.728593
GAGGTGC26650.065.6665343
GAGGTCG45100.065.4217763
GAGGTGA99700.065.331483
AGGTAGA60150.064.405544
AGGTAAG62850.064.367084
Produced by FastQC (version 0.11.5)