FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578265
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG557619.642810822027963No Hit
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT23540.4070797990540669Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA20780.359350816667099Illumina Single End PCR Primer 1 (97% over 35bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT18740.32407287316368794Illumina Single End PCR Primer 1 (97% over 35bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA11750.20319403733582353Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT8570.14820194893344746Illumina Single End PCR Primer 1 (97% over 35bp)
ACGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA8040.1390366008663848Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT444150.069.8174061
CCGCATA177950.069.6936652
CCGCATT100750.069.348262
CGCATAA58650.069.3398743
CCGCATG73050.069.241712
CGCATAG37050.069.2405853
CCGCATC95750.069.204122
CGCATAC24350.068.990173
CGCATAT61200.068.623813
CGCATTC16600.068.309623
CGCATCA34000.068.2463763
CGCATTG21600.068.2139743
CGCATGG15700.068.212943
CGCATGT22150.068.100223
CGCATGA23250.067.888863
CGCATTA28050.067.875183
CGCATTT37450.067.4730453
CGCATCT32800.067.435433
CGCATCC19100.067.2477343
CGCATCG12700.066.413793
Produced by FastQC (version 0.11.5)