FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578265
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG557619.642810822027963No Hit
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT23540.4070797990540669Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA20780.359350816667099Illumina Single End PCR Primer 1 (97% over 35bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT18740.32407287316368794Illumina Single End PCR Primer 1 (97% over 35bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA11750.20319403733582353Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT8570.14820194893344746Illumina Single End PCR Primer 1 (97% over 35bp)
ACGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA8040.1390366008663848Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT444150.069.801641
CCGCATA177850.069.693482
CCGCATT100750.069.348262
CGCATAA58500.069.3381963
CGCATAG37200.069.243633
CCGCATC95750.069.204122
CCGCATG73100.069.194342
CGCATAC24400.068.992233
CGCATAT61050.068.620433
CGCATTC16600.068.309623
CGCATCA33950.068.24383
CGCATTG21700.068.2221763
CGCATGG15700.068.212943
CGCATGT22100.068.0959243
CGCATGA23300.067.893393
CGCATTA28000.067.871393
CGCATTT37400.067.469673
CGCATCT32750.067.4315263
CGCATCC19150.067.254913
CGCATCG12750.066.427843
Produced by FastQC (version 0.11.5)