FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YCol_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YCol_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702030
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290144.132871814594818No Hit
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGT9280.13218808313035055Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGC8930.12720254120194294Illumina Single End PCR Primer 1 (96% over 33bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGT8600.12250188738373005Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT405700.069.7587361
ACCGCAT216400.069.4837341
CCGCATA87400.069.446922
TGAGGTA188050.069.337522
CCGCATT49650.069.0206152
TGAGGTG62950.068.8399052
CGCATAA31200.068.661323
TGAGGTC88100.068.656742
CGCATTA14200.068.5285953
GAGGTAT63400.068.3512953
GAGGTAC20900.068.33283
CCGCATG35500.068.232792
GAGGTAA71850.068.205063
CGCATAG17850.067.654433
GAGGTCT33800.067.625713
CGCATAT30300.067.581623
GAGGTAG36500.067.4183043
CGCATCA17100.067.346523
GAGGTCA32150.067.1768343
CGCATAC10950.067.13063
Produced by FastQC (version 0.11.5)