FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YCol_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YCol_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702030
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290144.132871814594818No Hit
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGT9280.13218808313035055Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGC8930.12720254120194294Illumina Single End PCR Primer 1 (96% over 33bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGT8600.12250188738373005Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT405650.069.75871
ACCGCAT216400.069.467561
CCGCATA87300.069.406192
TGAGGTA188050.069.337522
CCGCATT49700.069.021612
TGAGGTG62900.068.8389742
CGCATAA31150.068.6591643
TGAGGTC88150.068.657512
CGCATTA14150.068.523373
GAGGTAT63350.068.349993
GAGGTAC20900.068.33283
GAGGTAA71750.068.2513353
CCGCATG35550.068.235282
GAGGTCT33650.067.615093
CGCATAT30250.067.5776143
CGCATAG17900.067.4654543
GAGGTAG36600.067.425383
CGCATCA17100.067.346523
GAGGTCA32200.067.181233
CGCATAC10900.067.11743
Produced by FastQC (version 0.11.5)