FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YCol_15YCol_16YCol_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YCol_15YCol_16YCol_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708341
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA884333.2652092184846735Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG593512.191415335070436No Hit
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA389221.437115931856439Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG77780.28718687934791076Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT54340.20063943203606932Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATA34850.12867655882327964Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT31130.11494121308948911Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT525100.069.490671
ACTCTAT1232450.069.370691
GTGAGGT543300.069.289441
TGAGGTA253500.068.341532
GATCGTA215850.068.293352
CTCTATA558900.067.669922
ACCGCAT162800.067.400331
CCGCATA66000.067.360452
CTCTATG181500.066.868632
GATCGTC109650.066.756042
CTCTATT308450.066.6190642
TGAGGTC115000.066.359612
CTCTATC226650.066.336512
GATCGTG97150.066.2649842
GATCGTT122050.066.255112
TGAGGTG91350.066.0270542
GAGGTAT87850.065.699173
GAGGTAA102650.064.921563
CCGCATT38100.064.775282
ATCGTAG41450.064.006743
Produced by FastQC (version 0.11.5)