FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YCol_15YCol_16YCol_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708341
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA884333.2652092184846735Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG593512.191415335070436No Hit
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA389221.437115931856439Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG77780.28718687934791076Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT54340.20063943203606932Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATA34850.12867655882327964Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT31130.11494121308948911Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT525350.069.504351
ACTCTAT1231850.069.381831
GTGAGGT543600.069.289931
GATCGTA215650.068.340542
TGAGGTA253750.068.3294752
CTCTATA559150.067.6522752
ACCGCAT162850.067.422721
CCGCATA66250.067.3705442
CTCTATG181750.066.873042
GATCGTC109500.066.71972
CTCTATT308400.066.652662
TGAGGTC115250.066.3982
CTCTATC225750.066.3374562
GATCGTG97700.066.286172
GATCGTT122000.066.282352
TGAGGTG91400.066.029332
GAGGTAT88000.065.825913
GAGGTAA102850.064.9315263
CCGCATT38100.064.7753752
ATCGTAG41150.064.303343
Produced by FastQC (version 0.11.5)