FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YC24_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YC24_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315093
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG930747.077370193590872No Hit
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGT35120.2670533566827593Illumina Single End PCR Primer 1 (97% over 34bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCC30590.2326071236026654Illumina Single End PCR Primer 1 (97% over 34bp)
GTGAGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAG26370.20051813826094428Illumina Single End PCR Primer 1 (97% over 34bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGT26070.1982369307721963Illumina Single End PCR Primer 1 (97% over 34bp)
GTGAGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAGG23600.17945498911483826Illumina Single End PCR Primer 1 (100% over 23bp)
CACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAG21530.16371465744247746Illumina Single End PCR Primer 1 (97% over 34bp)
GTGAGGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAG19370.14728996352349225Illumina Single End PCR Primer 1 (100% over 22bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTG16160.12288104339388925Illumina Single End PCR Primer 1 (97% over 34bp)
GTGAGGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGG13450.10227413574553283Illumina Single End PCR Primer 1 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT658950.069.7491761
CCGCATA272550.069.600732
CCGCATT146550.069.521172
GTGAGGT412350.069.491641
CCGCATG106600.068.948182
CGCATAG57150.068.771383
CGCATAA90300.068.67843
TGAGGTA188150.068.659482
CGCATAT94300.068.585843
CCGCATC140350.068.552472
CGCATAC36950.068.1965263
TGAGGTC88350.067.9784852
CGCATTA41150.067.784863
CGCATCA49000.067.781993
CGCATTG35100.067.7028353
CGCATGT33700.067.296013
CGCATTC24700.067.0206153
CGCATGG24550.066.859873
CGCATTT51200.066.85183
GAGGTAT66850.066.802623
Produced by FastQC (version 0.11.5)