FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568567
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1196287.626578909284716No Hit
GGATCGATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA116920.7453937256107007Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT53200.33916307049682926Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT45850.2923050147045042Illumina Single End PCR Primer 1 (96% over 33bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT44790.2855472542773117Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG28690.18290579873221866Illumina Single End PCR Primer 1 (96% over 33bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC24140.15389843086077928Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG20620.1314575660459515Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT18350.11698575833866197Illumina Single End PCR Primer 1 (96% over 33bp)
GGACGATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC16650.10614784067240991Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA401450.069.727082
ACCGCAT979350.069.695061
GGATCGT277900.069.545211
CCGCATT218050.069.4442442
CGCATAT134050.069.087773
CCGCATG159950.068.802492
CGCATAA132100.068.782833
CGCATAG86850.068.712013
CGCATTA61000.068.452413
CCGCATC208750.068.396372
GATCGTA113000.068.271422
CGCATAC56350.067.95193
CGCATTC39950.067.7237243
CGCATGA49700.067.4663543
CGCATCA75000.067.43493
CGCATGG32950.067.346023
GATCGTT66150.067.254532
CGCATTT75950.066.729663
CGCATGT53600.066.6713263
CGCATCT73900.066.591543
Produced by FastQC (version 0.11.5)