FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_SPL9_13YCol_14YC24_15YCOL_16YC24_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568567
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1196287.626578909284716No Hit
GGATCGATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA116920.7453937256107007Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT53200.33916307049682926Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT45850.2923050147045042Illumina Single End PCR Primer 1 (96% over 33bp)
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT44790.2855472542773117Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG28690.18290579873221866Illumina Single End PCR Primer 1 (96% over 33bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC24140.15389843086077928Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG20620.1314575660459515Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT18350.11698575833866197Illumina Single End PCR Primer 1 (96% over 33bp)
GGACGATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC16650.10614784067240991Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA401050.069.726812
ACCGCAT979450.069.691521
GGATCGT277850.069.545121
CCGCATT217950.069.4600452
CGCATAT134200.069.088793
CCGCATG160200.068.804372
CGCATAA131800.068.7534943
CGCATAG86900.068.712753
CGCATTA61100.068.397673
CCGCATC209000.068.381552
GATCGTA113250.068.275252
CGCATAC56100.067.942763
CGCATTC39950.067.7237243
CGCATGA49650.067.4638063
CGCATCA74950.067.433193
CGCATGG33250.067.369983
GATCGTT65750.067.131322
CGCATGT53450.066.7929463
CGCATTT75900.066.727513
CGCATTG49650.066.547363
Produced by FastQC (version 0.11.5)