FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_HALObZIP63_pGSTbZIP53_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_HALObZIP63_pGSTbZIP53_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644674
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13620.21126957190766185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1000.045.4961626
GCGGACT400.002419646634.9970475
ACCGGGC450.00431212231.10365936
GGACTGT1554.3655746E-1127.0944927
GACTGTA1554.3655746E-1127.0944928
ACTGTAG2207.2759576E-1222.270859
CTGTAGA2603.6379788E-1220.19060710
GGACAGG900.005291196519.43375268
ACAGTCC1501.4908939E-518.67234225
GGACACG1150.001008458418.25791462
GTGACGT1000.00968192417.50124232
CACTGAT1854.747035E-617.0255938
TCGGACT1250.001762451416.7985845
GGTGGTC1250.001767952216.79076269
ACGGACT1906.1182636E-616.577555
GCTGGTG1704.3783926E-516.47175858
CTGGTGC1400.003749763115.00106359
GTAGAAG2858.524148E-814.735599512
GTGGTCG1500.0059531613.99230170
GGGATCA1600.00908146713.12695116
Produced by FastQC (version 0.11.5)