FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_HALObZIP53_pGSTbZIP63_Col_B_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_HALObZIP53_pGSTbZIP63_Col_B_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11247735
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128670.11439636513484715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGC8753.5166267E-66.799703665
ACGCGGG16156.002665E-116.2851982
CCGCGCT10104.877744E-66.23721366
GCGACGC18955.875336E-105.539891768
CGACGCA21706.6211214E-105.160330369
CGCGGGC22754.456524E-105.0769953
Produced by FastQC (version 0.11.5)