FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_HALObZIP53_pGSTbZIP63_Col_B_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_HALObZIP53_pGSTbZIP63_Col_B_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11247735
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128670.11439636513484715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGG15755.456968E-126.6671882
CCCGCGC8952.3852426E-45.86562465
CCGCGCT10202.3490896E-45.489795766
GCGACGC19157.694325E-105.482043368
CGCGGGC22153.8198777E-115.3725943
GACGCGG24857.2759576E-125.2128811
CGACGCA21757.039489E-105.148569
Produced by FastQC (version 0.11.5)