FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_16YCol_Col_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_16YCol_Col_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000310
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180760.3012511020263953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT5719750.069.88531
GATCGTA2242300.069.516762
GATCGTC1201550.069.211432
GATCGTT1226050.069.127352
GATCGTG1111400.069.0243452
ATCGTAG438300.068.3244863
ATCGTAA809000.068.309823
ATCGTAT745950.067.9744643
ATCGTGC145350.067.6656653
ATCGTAC314900.067.545063
ATCGTGT373350.067.385893
ATCGTGA399450.067.3552553
ATCGTCA439950.067.0260543
ATCGTGG229950.066.789823
ATCGTCT430800.066.727243
ATCGTTG313850.066.633533
ATCGTTA339050.066.408983
ATCGTCC226850.065.557893
ATCGTTT439300.065.412233
ATCGTTC201550.065.243233
Produced by FastQC (version 0.11.5)