FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_16YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_16YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000310
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180760.3012511020263953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT5719550.069.883041
GATCGTA2243950.069.515142
GATCGTC1201650.069.228552
GATCGTT1224650.069.125932
GATCGTG1110000.069.028992
ATCGTAA811450.068.323143
ATCGTAG438600.068.277333
ATCGTAT746200.067.979423
ATCGTGC144500.067.6030653
ATCGTAC313650.067.557183
ATCGTGT372200.067.3773963
ATCGTGA401000.067.338893
ATCGTCA439900.067.0332643
ATCGTGG229100.066.884443
ATCGTCT430550.066.724943
ATCGTTG315050.066.634853
ATCGTTA337050.066.366493
ATCGTCC226900.065.57393
ATCGTTT438350.065.441813
ATCGTTC201650.065.2972643
Produced by FastQC (version 0.11.5)