FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_15YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_15YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150236
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT45599514.47494727379155Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT2916749.2587983884382Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2557418.118153687533251No Hit
CTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA415291.3182821858425846Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA394861.2534299017597412Illumina Single End PCR Primer 1 (97% over 35bp)
ATCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA246680.7830524443248061Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAT179210.5688780142186173Illumina Single End PCR Primer 1 (97% over 34bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGGAT132770.42146048740475317Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAT97890.3107386240268983Illumina Single End PCR Primer 1 (97% over 34bp)
ATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGT75980.24118827922733407Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA75950.24109304826685998Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGGAT73990.23487129218255393Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGT70590.22407844999549237Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG70320.22322137135122574Illumina Single End PCR Primer 1 (97% over 35bp)
ACTTAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT64180.20373076810753227Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGGAT61480.19515998166486573Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTTTAGGAT58530.18579560388491528Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAA47380.15040143024205171Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGACTGTAGGAT44560.1414497199574889Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGAT36540.1159913098574202Illumina Single End PCR Primer 1 (97% over 34bp)
ACTCTATAGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA35350.11221381509194867Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGGAT34280.10881724416837342Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTTTAGGAT32000.10157969117234392Illumina Single End PCR Primer 1 (97% over 35bp)
ACTCAATAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAA31530.10008773945825011Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT2183200.069.6114041
CTCTATG283250.068.559542
CTCTATT401800.068.393752
CTCTATC330950.068.37662
CTCAATA399750.068.149062
TCAATAG404050.066.89433
CTCTATA1242700.066.4732742
TCTATAA187850.065.979523
CAATAGA411950.065.682874
ACTCAAT422000.065.530551
TCTATAT206000.065.127753
TCTATCA120950.065.0556263
TCTATAC87800.065.0210343
TCTATGA104900.064.598783
TCTATCC58800.064.587263
TCTATGT96700.063.742243
TCTATTG90950.063.654233
TCTATCT124200.063.4660153
ACTCTAA70700.063.2842371
TCTATTA119450.063.1474153
Produced by FastQC (version 0.11.5)