FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_14YCol_Col_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_14YCol_Col_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344582
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365431.0926029022460804No Hit
CACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG143140.4279757530238458Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAGG65880.19697528719582894Illumina Single End PCR Primer 1 (100% over 23bp)
GTGAGGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG46170.13804415619051946Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCT43000.12856614070158842Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAG41100.12288531122872755Illumina Single End PCR Primer 1 (100% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT3045500.069.832481
TGAGGTA1343950.069.461382
TGAGGTC690200.069.1638642
TGAGGTG518300.068.7293242
GAGGTAA514900.068.54893
GAGGTAT437350.068.539043
TGAGGTT532100.068.157232
GAGGTCT261000.068.032253
GAGGTAG268750.067.750493
GAGGTAC154000.067.7080463
GAGGTCA251450.067.3588263
GAGGTCC109700.067.259633
GAGGTCG88200.066.5510563
GAGGTGT197800.066.358343
GAGGTTA179000.066.0928043
GAGGTTG157350.065.421243
GAGGTGA196250.065.241593
GAGGTGC49550.064.917593
AGGTAAG122400.064.87814
AGGTAGA113650.064.699614
Produced by FastQC (version 0.11.5)