FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_14YCol_Col_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_14YCol_Col_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344582
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365431.0926029022460804No Hit
CACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG143140.4279757530238458Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAGG65880.19697528719582894Illumina Single End PCR Primer 1 (100% over 23bp)
GTGAGGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG46170.13804415619051946Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCT43000.12856614070158842Illumina Single End PCR Primer 1 (96% over 33bp)
GTGAGGGTCTGAACTCCAGTCACCTCACAGATCGGAAGAGCGTCGTGTAG41100.12288531122872755Illumina Single End PCR Primer 1 (100% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGGT3045450.069.836751
TGAGGTA1345300.069.4628452
TGAGGTC689450.069.17912
TGAGGTG518100.068.7162252
GAGGTAT437850.068.557613
GAGGTAA516400.068.5404743
TGAGGTT531650.068.156562
GAGGTCT260350.067.98793
GAGGTAG267950.067.809973
GAGGTAC153800.067.7287143
GAGGTCA251050.067.425213
GAGGTCC109950.067.266773
GAGGTCG88400.066.480573
GAGGTGT197400.066.440493
GAGGTTA179500.066.007083
GAGGTTG157500.065.426473
GAGGTGA195500.065.224193
GAGGTGC49950.064.88913
AGGTAAG122350.064.848274
AGGTAGA112900.064.7892764
Produced by FastQC (version 0.11.5)