FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n02_13YCol_Col_lib_k.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n02_13YCol_Col_lib_k.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760927
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22466112.758109791036198No Hit
ATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT72850.41370255552899127Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA66120.3754840490264503Illumina Single End PCR Primer 1 (97% over 35bp)
ACATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA39370.2235754236263059Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT32840.1864926825473174Illumina Single End PCR Primer 1 (97% over 35bp)
ACGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA29200.16582175183866224Illumina Single End PCR Primer 1 (97% over 35bp)
ACCGCATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT25160.14287929028290214Illumina Single End PCR Primer 1 (97% over 35bp)
ACCAATGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA18590.10556939611920313Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA531950.069.789342
ACCGCAT1386600.069.7487261
CCGCATT304350.069.6088942
CGCATAG120100.069.329613
CGCATAA167550.069.268763
CCGCATG239250.069.136772
CGCATAT170400.069.096133
CGCATTA77250.068.821893
CGCATAC81900.068.80333
CCGCATC321300.068.7362752
CGCATTC61150.068.053853
CGCATGG51900.068.0442053
CGCATGT78800.067.956743
CGCATTG75750.067.828283
CGCATCA110800.067.693933
CGCATTT99800.067.40473
CGCATGA74900.067.242883
CGCATCG46900.067.23873
CGCATCT114550.066.7305763
CGCATCC59400.066.6413043
Produced by FastQC (version 0.11.5)